In separate test tubes, prepare 2mL of 0.2 M of the following buffer solutions by mixing volumes of 0.1 M salt and 0.1 M acid/base

Test tube Number

Buffer solution

Salt

Acid/Base

1

Acetate buffer

0.1 M CH3COOH

0.1 M CH3COOH

2

Phosphate buffer

0.1 M Na2HPO4

0.1 Na2HPO4

 

QUESTION:

The internal pH of a muscle is 6.8. Calculate the [H2PO4-]/[HPO42-] ratio in the cell. The second dissociation constant of phosphoric acid is 6.31×10-8

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